Trending PCR Discussions

How can i Analyse the results of RT-PCR?‎

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Haw can you measure alcohol in blood? ‎

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There is a method to clone a gene without the use of restriction enzymes. Anyone here knows the strategy? It's a primitive technique (hint)!‎

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What is the difference between conventional PCR and Real time PCR? ‎

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What is the principle of real time PCR? ‎

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Answer added by  Kaleem Iqbal, Research Assistant, King Faisal Specialist Hospital
8 years ago

1. Conventional PCR gives result only at the end of the reaction while Real Time PCR displays the data of amplification during the run after each cycle. 2. Real Time PCR ... See More

 

Answer added by  susan kamalizeni, Medical Laboratory Scientist, Mbabane Government Hospital,National Reference Laboratory
6 years ago

With a Real time PCR, Detection and Amplification are done on the same instrument.

 

Answer added by  HENNA ROHIYA, Technician, Tissue Typing and Molecular Biology DepartmentKing Salman Armed Forces Hospital
7 years ago

Actually you need qPCR Ct values (raw data) for the following: the housekeeping gene: control and experimental conditions the gene being tested: control and experimental ... See More

 

Answer added by  Muhammad Ammar Athar, Postdoctoral fellow/Foreign Expert, Pharmaceutical College of Henan University
7 years ago

1.     Conventional PCR is more time consuming as it uses gel electrophoresis to analyze the amplified PCR products. In contrast, real-time PCR is less time consuming as ... See More

 

Answer added by  Vesna Mandusic , Research professor, Vinca Institute of Nuclear Sciences
6 years ago

There are two ways: the delta delta Ct method and the standard curve method (relative or absolute standard curve). Accordingly, the analysis will give the relative values ... See More