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according to the expected size of the fragment that we will obtain and the total size of the organism's genome it' ranges from100bp to1 kbp
what range you are expecting will depends on your sample. I guess min50bp to max10kb range of markers available.
There are different methods like: Toposiomerase technology,Sequence and ligase independent cloning,
pour cloner un gêne sans utilise des enzymes de restriction , on peut se référer a une méthode simple , nommé la technologie des topoisomerases , : La technolo ... See More
Gene can be cloned using PCR (polymerase chain reaction) technique. In this technique copies of fragmented DNA is made using PCR. Those fragmented DNAs are used for makin ... See More
Well - Ethical implications comes into the picture when one carries out research using embryo's (specifically embryonic stem cells). For this kind of research aborte ... See More