Discussions Populaires Dans la Communauté PCR

How can i Analyse the results of RT-PCR?‎

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Haw can you measure alcohol in blood? ‎

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There is a method to clone a gene without the use of restriction enzymes. Anyone here knows the strategy? It's a primitive technique (hint)!‎

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What is the difference between conventional PCR and Real time PCR? ‎

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What is the principle of real time PCR? ‎

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La répondre a été ajoutée par  Kaleem Iqbal, Research Assistant, King Faisal Specialist Hospital
Depuis 9 années

1. Conventional PCR gives result only at the end of the reaction while Real Time PCR displays the data of amplification during the run after each cycle. 2. Real Time PCR ... Voir Plus

 

La répondre a été ajoutée par  susan kamalizeni, Medical Laboratory Scientist, Mbabane Government Hospital,National Reference Laboratory
Depuis 6 années

With a Real time PCR, Detection and Amplification are done on the same instrument.

 

La répondre a été ajoutée par  HENNA ROHIYA, Technician, Tissue Typing and Molecular Biology DepartmentKing Salman Armed Forces Hospital
Depuis 7 années

Actually you need qPCR Ct values (raw data) for the following: the housekeeping gene: control and experimental conditions the gene being tested: control and experimental ... Voir Plus

 

La répondre a été ajoutée par  Muhammad Ammar Athar, Postdoctoral fellow/Foreign Expert, Pharmaceutical College of Henan University
Depuis 7 années

1.     Conventional PCR is more time consuming as it uses gel electrophoresis to analyze the amplified PCR products. In contrast, real-time PCR is less time consuming as ... Voir Plus

 

La répondre a été ajoutée par  Vesna Mandusic , Research professor, Vinca Institute of Nuclear Sciences
Depuis 6 années

There are two ways: the delta delta Ct method and the standard curve method (relative or absolute standard curve). Accordingly, the analysis will give the relative values ... Voir Plus