What is the difference between normal and reverse phase chromatography?

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Question ajoutée par safia islahi
Date de publication: 2016/10/09

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par Ankita Gambhir , Lab Specialist , Scientific Diamond Company

Normal Phase Chromatography

Stationary Phase : Polar

Mobile Phase: Non-Polar

order of elution- Non-Polar to Polar

Reverse Phase Chromatography:

Stationary Phase : Non-Polar

Mobile Phase: Polar

order of elution- Polar to Non-Polar

Why in normal stationary phase the principle of separation is mainly adsorption while in reversed phase it's partition ?
Top Answer: In case of Normal Phase. In a non-polar environment, hydrophilic molecules will tend to associate wit ... Voir Plus

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Make the distinction between reverse phase and normal phase explain and give the reasons, which one uses to separate hydrocarbons?
Top Answer: Reverse phase is non polar stationary phase and polar mobile phase and normal phase is the opposite, you ... Voir Plus

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