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You regenerate HPLC columns by flushing it with the following volumes of solvent. 20 column volumes of water/acetonitrile (95:5 v/v) 20 column volumes of acetonitrile 5 ... See More
by multiple washing with mobile phase they can be regenerate
HPLC columns is regenerate by washing washing with purified water and preserve with methanol or Acetonytrle 50% depend open the type of mobile phase we are using
To clean and regenerate a reversed phase column flush it with the following volumes of solvent. water/acetonitrile (95:5 v/v) acetonitrile isopropanol hexane isopropa ... See More
BY PASSING 1 M NACL SOLUTION THROUGH CHROMATOGRAPHY COLUMN.
For HPLC Reverse phase; wash with unbuffered mobile phase wash with100% Water wash with Methaol or ACN wash with THF or IPA wash with Methylene chloride wash with N-Hepta ... See More
regeneration done by organic solvent like acetonitril
For Ion Chromatographic columns, you can regenerate your column by one the following methods:- 1. Hydrophilic contamination of low valence: Concentrated eluent of10 times ... See More