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Thank you for the question, firstly though -scopy and -metry are often used interchangeably In my opinion the term spectroscopy should be more appropriate here. Now to the question, popular techniques in this filed are UV, IR & NMR. Of these UV can be applied to any sample with sufficient conjugation/ heteratom so as the absorption occurs in the UV region of the EM spectum. Similarly IR can be employed for FG identification, etc. But both of these are preliminary techniques and when applied to biochemistry require thorough sample clean up procedures.
Spectrophotometry measure sthe absorption of light by a substance. In biochemistry, it can give the concentration of a DNA or RNA sample and allows to determine the purity of DNA and RNA of a preparation (after isolation for example).
Many kinds of molecules interact with or absorb specific types of radiant energy in a predictable fashion. For example, when while light illuminates an object, the color that the eye perceives is determined by the absorption by the object of one or more of the colors from the source of the white light. The remaining wavelength(s) are reflected (or transmitted) as a specific color. Thus an object that appears red absorbs the blue or green colors of light (or both), but not the red.
These include acid/base chemistry, energetics, enzyme kinetics, spectrophotometry, and isotopes
To determine the impurities in food or any other things
For color measurement. to the determine the concentration.
on utilise la spectroscopie pour connaitre le type des molécule
Quantitative assay for protein, Enzyme Assays, Enzyme kinetics, ligand binding reactions .
predicting the concentrations and purity of molecules
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