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Bakeout, reverse flow, rinsing with solvent and trimming
Washing with water to remove blocking which were soluble in water, fallowed by organic solvent either Acetonitrile or Alcohol/Methanol, then mild acidified organic solution , and finally washing for Overnight
* trimming 50cm of the front end of the column and recalculate the linear flow rate.
* rinsing the column with solvent such as pentane, polar columns: methanol).
3. reversing the flow by: - installing the column detector end in the inlet.
Continous flowing with carrier gas only, with recommended pressure and temperature oven.
There are several methods for GC column regeneration: 1. trimming 50cm of the front end of the column, then we have to recalculate the linear flow rate.
2. rinse the column with solvent by forcing the appropriate solvent to go through the column from the detector end (non-polar columns: pentane, polar columns: methanol).
3. reversing the flow by: - installing the column detector end in the inlet.
- heating the column, without exceeding the maximum temperatures mentioned by the column manufacturer.
Generally there are two types of columns. Packed and Capillary. The term regeneration in current analytical applications which are more standardized is nothing but to get rid of saturated analytes from the stationary phase. To achieve this simple understanding of the column is required.
1. The maximum temperature the column can withstand ( helps us not to cook the column)
2. Maximum flow the column could withstand - Mesh size or ID ( helps us not to bleed the column)
if you stay just 10 percent lower to the maximum temperature and flow and use proper mobile phase for a extended period of time( say 12~24 hrs, depending on the stationary phase), the column can be regenerated. This is more like a thumb rule. However there are some speciality columns where you can always ask the manufacturer for specific regeneration requirements and do so.
Hope this clarifies.
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