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Mycobacterium tuberculosis is called acid-fast because:

<p><strong><span>a) It is easily stained by gram stain</span></strong></p> <p><strong><span><span>b) Once stained, it can not be remove by acid</span></span></strong></p> <p><strong><span>c) It is easily decolorizes after staining.</span></strong></p> <p><strong><span>d) It is stained by an alkaline stain</span></strong></p> <p><strong><span>e) None of the above</span></strong></p> <p> </p> <p><span><strong>f) All of the above</strong></span></p>

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Question added by mohammad ali , رئيس قسم المختبرات , مؤسسة متحده التطوير
Date Posted: 2014/11/08
Mohamed  shaheen
by Mohamed shaheen , مدرس كلينيكال باثولوجي , كلية الطب جامعة الأزهر

  • Initially, Carbol Fuchsin stains every cell
  • When they are destained with acid-alcohol, only non-acid-fast bacteria get destained since they don't have a thick, waxy lipid layer like acid-fast bacteria.
  • When counter stain is applied, non-acid-fast bacteria pick it up and become blue when viewed under the microscope. Acid-fast bacteria retain Carbol Fuchsin so they appear red.

Mozfar Idrees
by Mozfar Idrees , Medical Laboratory Director , Asia Polyclinic

resistance to decolorization by acids

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