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What is the basic principle for Uv Visible spectrophotometer?

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تم إضافة السؤال من قبل Ankita Gambhir , Lab Specialist , Scientific Diamond Company
تاريخ النشر: 2017/02/19
Paradzai Mugabazi
من قبل Paradzai Mugabazi , Educator , Department of Education

Based on Beer lambert s law for the relationship between absorbance and the substrate concentration for quantitative analysis 

Parthasarathi S
من قبل Parthasarathi S , Lab technician , Schlumberger kuwait contract

 

UV visible spectroscopy owrking based on The Beer-Lambert law  is the linear relationship between absorbance and concentration of an absorbing species.

Sara Arafeh
من قبل Sara Arafeh , Office Assistant , University of Wisconsin Oshkosh

Absorption of UV or visible light. Conjugated systems like a benzene ring absorb UV light efficiently 

Mohd Nadeem  Shah Syed Bukhari
من قبل Mohd Nadeem Shah Syed Bukhari , Assistant professor , Islamic University of science and technology

The basic principle of UV visivle spectroscopy is that the absorption of light of a solution is dirrectly proportional to the concentration of that solution.

HALYMA WASABO
من قبل HALYMA WASABO , WASH & QUALITY ASSISTANT , PEACE WINDS JAPAN

The amount of light absorbed by a solution is directly proportional to the concentration of the solution.

mohd faeek mohd faeek
من قبل mohd faeek mohd faeek , QC Officer , Industrial training from

The Basic Principleof UV-Vis Spectrophotometer is based on the beer's Lambert low. In which a beam of light is passed through the solution absorb the monochromatic radiation of solution by a substance.

TAOUFIK GHARBI
من قبل TAOUFIK GHARBI , DIRECTEUR GENERAL , LAB SYSTEMS

it's a moleculaire spectroscopie technic in UV and Visible wevelenghth range from about 190 to 1100nm )used for qualitiative ( scan and wevlegth methode for spetre determination and identification of molecule )and quantitative methode when coumpound are identified and need to be quantifyed ( calibration and mesure )

Chaka Chawasarira
من قبل Chaka Chawasarira , Senior Laboratory Technician , Strata Solvay Advanced Materials

the principle is based on the Beer-Lambert law which states that when a beam of monochromatic light is passed through a solution of an absorbing substance the rate of decrease pf intensity of radiation with thickness of the absorbing solutin is proportional to the incident radiation as well as the concentration of the solution

A=log(Lo/L)=EcL from the expression of Beer- Lambert law

ABDUL GAFUR SHAIK
من قبل ABDUL GAFUR SHAIK , Junior Manager , APOTEX PHARMACHEM INDIA Ltd

UV/Visible Spectroscopy is based on the principle A=epsilon.ct which is comes from  Lambert and beers law of absorption(A) . Absorbed light is directky proportional to (species present in the test solution) concentration and thickness of the medium .

Muhammad Awais
من قبل Muhammad Awais , Quality control Officer , Himont Pharmaceutical Pvt Ltd.

UV/Visible Spectroscopy works on Lambert beer law of absorption . Absorbed light is directky proportional to concentration of the species present in the test solution.

rim  ben debabis
من قبل rim ben debabis , in the laboratory of research , GCT

UV spectroscopy is type of absorption spectroscopy in which light of ultra-violet region (200-400 nm.) is absorbed by the molecule. Absorption of the ultra-violet radiations results in the excitation of the electrons from the ground state to higher energy state. The energy of the ultra-violet radiation that are absorbed is equal to the energy difference between the ground state and higher energy states (deltaE = hf)

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