What are critical in plant tissue culture..????

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Afnan Said Abdallah Zuiter
04-March-2014

Plant Tissue Culture

Question added by Sabeetha Senevirathne , Research Assistant , Field Crops Research and Development Institute -DOA
Date Posted: 2014/10/06

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3 Answers

MUHAMMAD ANWAR MALLICK

by MUHAMMAD ANWAR MALLICK , Director, Biotechnology & Head, Department of Botany , VINOBA BHAVE UNIVERSITY,HAZARIBAGH

A suitable culture medium with growth Regulators/Extracts

Culture Room with controlled conditions for proper growth.

Sterile/Aseptic conditions to transfer the Explants in the culture medis

Sterile Explants

Proper instruments/Chemicals/Reagents etc.

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Abdullah Al Hinidi Al Hinidi

by Abdullah Al Hinidi Al Hinidi , COMMERCIAL OPERATOR , RENFE

Appropriate tissue
A suitable growth medium
sterile conditions
Growth regulators
Frequent subculturing

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Mohd Fahad Parvez

by Mohd Fahad Parvez , QA/QC Executive , Allanasons Pvt Ltd

1. A suitable growth medium,2. Aseptic condition,3. Growth regulators,4. Sterilization5. Culturing (Micropropogation) plant tissue.

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